RetrovirusiPSCReprogrammingKit:#RF101
ProductDescription
| ProductSpecifications | |
| ProductName | RetrovirusiPSCReprogrammingKit |
| Catalog# | RF101 |
| Content | Retroviruscocktailcontaining:humanOct4retrovirus(cat#RF01O)2vials,20μleachhumanSox2retrovirus(cat#RF01S)2vials,20μleachhumanKlf4retrovirus(cat#RF01K)2vials,20μleachhumanc-Mycretrovirus(cat#RF01M)2vials,20μleachGFPretrovirus(cat#RF01G)1vial,20μleachTransPlusvirustransductionenhancer(cat#V020)1vial,100μl |
| Shipping | DryIce |
| StorageandStABIlity | Storeat-80°C.Thisproductisstableupto6monthswhenstoredasdirected. |
| QualityControl | EachlotofHumaniPSCellReprogrammingRetrovirusKitistestedforsterilityandsuccessfulreprogramming. |
| RestrictedUse | ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures. |
| Protocol |  Download |
- Overview
- Procedure
Humaninducedpluripotentstemcells(iPSCs)canbederivedfromsomaticcellsthroughareprogrammingprocessdrivenbyectopicexpressionofadefinedsetofreprogrammingfactors:Oct4,Sox2,Klf4andc-Myc.ThesehiPSCssharethepropertiesofself-renewalandpluripotencywithhumanEScells,andcanthereforebeusedasarenewablesourceforalldifferentiatedcelltypesofthebody.HumaniPSCscanbegeneratedfrompatientsofvirtuallyanygeneticbackground.
Retrovirusesareefficienttoolsfordeliveringheritablegenesintothegenomeofdividingcells.TheVSV-Gpseudotypedretrovirushasawiderangeoftargetsincludingbothmammalianandnon-mammaliancells,andisusuallysilencedinEScells.TheretrovirusiscommonlyusedingeneratingiPSCsbecauseofitshighreprogrammingefficiency.TheHumaniPSCellReprogrammingRetrovirusKitofferssuchopportunitytogenerateiPSCsfromvarioustissuesandcelltypes.
Materialsyouwillneed:
HumanESmedium
| DMEM/F12 | Invitrogen | 10565-042 | ||
| Pen/strep | Invitrogen | 15140-122 | 1X | 1mL |
| L-Glutamax | Invitrogen | 35050-061 | 2mM | 2mL |
| nonessentialaminoacids | Invitrogen | 11140-050 | 0.1mM | 2mL |
| 2-mercaptoethanol | Sigma | M7522 | 0.1mM(1000x) | 0.2mL |
| knockoutserumreplacement | Invitrogen | 10828-028 | 20% | 40mL |
| bFGF | StemRD | bFGF-050 | 10ng/mL | 0.2mL |
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